First and Second Derivative Spectrophotometric Methods for Determination of Olanzapine in Pharmaceutical Formulation

Simple, fast and reliable derivative spectrophotometric methods were developed for determination of olanzapine in bulk and pharmaceutical dosage forms. The solutions of standard and the sample were prepared in methanol. The quantitative determination of the drug was carried out using the first derivative values measured at 222 nm and the second derivative values measured at 230 nm (n=6). Calibration graphs constructed at their wavelengths of determination were linear in the concentration range of olanzapine using 2-10 μg/ml for first and second derivative spectrophotometric method. The calibration graphs constructed at their wavelength of determination were found to be linear for UV and derivative spectrophotometric methods. All the proposed methods have been extensively validated as per ICH guidelines. There was no significant difference between the performance of the proposed methods regarding the mean values and standard deviations. Developed spectrophotometric methods in this study are simple, accurate, precise, specific, sensitive, reproducible and can be directly and easily applied to pharmaceutical dosage form.